Mycobacteria Real-time PCR Detection Kit

Mycobacteria Real-time PCR Detection Kit can detect tuberculosis and nontuberculous mycobacteria simultaneously in one tube. It employs quantitative real-time analysis of DNA samples from various sources based on the unique pairs of probes and optimized buffers. The kit shows a high coincidence with traditional methods, but with analysis in 3 hours rather than days.

Key Features| Principle| Specifications| Applications| Ordering Information| Certification| Reference|

◆ Accuracy: TB and NTM can be detected simultaneously in one tube with parallel, real-time fluorescence data
◆ Sensitivity: Specially designed probes ensure the high sensitivity and specificity. The lower detection limit is 10 MTB or 100 NTM bacteria per PCR reaction
◆ Efficiency: One test one tube format for easy and rapid detection of both MTB and NTM.
◆ A rapid assay which can be done in 3 hours
◆ Evaluation with hundreds of clinical samples shows a high coincidence with traditional methods






Packing Size
20 tests / kit
at -20 in darkness
Shelf Life
8 months
ABI 7000, ABI 7500, Eppendorf Mastercycler realplex 4
Down to 10 MTB or 100 NTM bacteria per PCR reaction

In the clinical trial of 1,071 samples by 3 independent hospitals, 97.0% of positive samples in bacteriologic analysis can be detected, the sensitivity is 50.3% (compared to about 10% for sputum smear and about 30% for sputum culture) and the specificity is 98.9%.

Cat. No.

Product Name

Product Description


Mycobacteria Real-time PCR Detection Kit

20 tests


CE-IVD Certificate

1. Tanil kocagoz, Engin yilmaz etc. Detection of mycobacterium tuberculosis in sputum samples by polymerase chain reaction using a simplified procedure. Journal of clinical microbiology, 1993, vol.31, No.6:1435-1438.
2. Eiman Mokaddas and Suhail Ahmad. Development evaluation of a multiplex PCR for rapid and differentiation of mycobacterium tuberculosis complex members from Non-Tuberculosis Mycobacteria. Japanese Journal of Infectious Diseases,2007, 60:140-144.
3. Sarman Singh, Krishnamoorchy Gopinath etc. Nontuberculous Mycobacterial infections in Indian AIDS patients detected by a novel set of ESAT-6 polymerase chain reaction primers. Japanese Journal of Infectious Diseases,2007, 60:14-18.
4. Tobin J.Hellyer, Lucky E DesJardin etc. Specificity of IS6110-Based amplification assays for Mycobacterium tuberculosis complex. Journal of clinical microbiology, 1996, Vol.34, No.11:2843–2846.
5. D. DE Wit, L. Steyn etc. Direct detection of Mycobacterium tuberculosis in clinical specimens by DNA amplification. Journal of clinical microbiology, 1990, Vol.28, No.11: 2437-2441.